The following diagram summarises the steps of an experiment similar to that carried out by Louis Pasteur, which identified microbes as agents of decay. Step 1 Two swan-neck flasks are filled partially with equal volumes of beef broth. Step 2 The broth is boiled for at least 20 minutes. Step 3 The neck of Flask P is left intact whereas the other is broken. Step 4 The flasks are observed two weeks later for evidence of decay.

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The following diagram summarises the steps of an experiment similar to that carried out by Louis Pasteur, which identified microbes as agents of decay - HSC - SSCE Biology - Question 5 - 2003 - Paper 1

Question 5

The following diagram summarises the steps of an experiment similar to that carried out by Louis Pasteur, which identified microbes as agents of decay. Step 1 Two s... show full transcript

Worked Solution & Example Answer:The following diagram summarises the steps of an experiment similar to that carried out by Louis Pasteur, which identified microbes as agents of decay - HSC - SSCE Biology - Question 5 - 2003 - Paper 1

Step 1

Which of the following statements best explains the results obtained?

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Answer

The correct answer is (D). Micorbes in Flask Q were not killed by boiling, and multiplied following the cooling down of the flask. In contrast, Flask P remained sealed due to its intact neck, preventing any microbes from entering, which is why no decay was observed there. This experiment demonstrates the importance of preventing microbial contamination in the preservation of substances.

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