The diagram represents some experimental steps used in the production of large amounts of human growth hormone - HSC - SSCE Biology - Question 19 - 2020 - Paper 1
Question 19
The diagram represents some experimental steps used in the production of large amounts of human growth hormone.
What makes this technology successful?
A. DNA ligas... show full transcript
Worked Solution & Example Answer:The diagram represents some experimental steps used in the production of large amounts of human growth hormone - HSC - SSCE Biology - Question 19 - 2020 - Paper 1
Step 1
A. DNA ligase can cut human growth hormone genes from human cells.
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Answer
This statement is incorrect. DNA ligase is an enzyme that joins together DNA fragments but does not cut them. The cutting of DNA is typically done by restriction enzymes.
Step 2
B. Human growth hormone can cause E. coli to grow and mature rapidly.
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This statement is misleading. While the human growth hormone can be produced in E. coli, it does not directly affect the growth rate of E. coli itself. The role of the hormone is rather in the production of desired proteins.
Step 3
C. Human plasmids containing the gene of interest can be inserted into bacteria.
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This statement is accurate. In biotechnology, human genes can be inserted into plasmids, which are then introduced into bacteria like E. coli. This allows for the production of human proteins, such as growth hormone.
Step 4
D. Restriction enzymes can produce sticky ends on both bacterial and human DNA.
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This statement is correct. Restriction enzymes cut DNA at specific sequences, creating sticky ends that facilitate the insertion of genes into plasmids, which can then be introduced into bacteria for protein expression.