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Yeast is a single-celled fungus that can reproduce by budding - HSC - SSCE Biology - Question 27 - 2019 - Paper 1
Question 27
Yeast is a single-celled fungus that can reproduce by budding. (a) What type of reproduction is budding? (b) Outline a procedure that could be used to test the eff... show full transcript
Worked Solution & Example Answer:Yeast is a single-celled fungus that can reproduce by budding - HSC - SSCE Biology - Question 27 - 2019 - Paper 1
Step 1
Step 2
Outline a procedure that could be used to test the effect of temperature on reproduction in yeast.
Answer
- Prepare a suspension of yeast cells.
- Prepare water baths at three different temperatures: 15°C, 25°C, and 35°C.
- Mix the yeast suspension thoroughly.
- Measure and place 5 mL of the suspension into 9 test tubes, distributing evenly.
- Place three test tubes in each water bath.
- Allow the yeast to incubate for a set period (e.g., 30 minutes).
- After incubation, take a drop from each test tube and place it on a microscope slide with a coverslip.
- Count the number of yeast cells in a given grid on the microscope slide to determine cell growth.
- Record the number of cells observed at each temperature.