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Four students were asked to design a first-hand investigation to determine the effect of pH on the activity of an enzyme - HSC - SSCE Biology - Question 4 - 2001 - Paper 1
Question 4
Four students were asked to design a first-hand investigation to determine the effect of pH on the activity of an enzyme. Their designs of the investigation are sho... show full transcript
Worked Solution & Example Answer:Four students were asked to design a first-hand investigation to determine the effect of pH on the activity of an enzyme - HSC - SSCE Biology - Question 4 - 2001 - Paper 1
Step 1
Which investigation is the most appropriate?
Answer
In examining the designs, Design B is the most appropriate as it consistently includes both the enzyme (E) and substrate (S) across varying pH levels, which allows for a direct assessment of the enzyme's activity relative to pH. This design has controlled the temperature, keeping it constant at 20 °C, eliminating other variables that could affect enzyme activity. It compares the activity of the enzyme at multiple pH levels, providing a comprehensive overview of its behavior under different conditions.