Jelly Bush honey has been used by Aboriginal Peoples to treat cuts, sores and burns - HSC - SSCE Biology - Question 26 - 2022 - Paper 1
Question 26
Jelly Bush honey has been used by Aboriginal Peoples to treat cuts, sores and burns. Recent studies have shown that Jelly Bush honey has a very high level of methylg... show full transcript
Worked Solution & Example Answer:Jelly Bush honey has been used by Aboriginal Peoples to treat cuts, sores and burns - HSC - SSCE Biology - Question 26 - 2022 - Paper 1
Step 1
Select appropriate safety equipment including gloves and safety clothing.
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Answer
It is essential to prioritize safety by using gloves and protective clothing while handling bacteria and Jelly Bush honey to prevent contamination and ensure safety.
Step 2
Prepare three nutrient agar plates, using sterile techniques. Seal one plate and label as control.
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Prepare and label the plates carefully, ensuring sterile techniques to avoid contamination. One plate will serve as the control, which will not have any Jelly Bush honey.
Step 3
Inoculate the remaining plates with bacteria to cover the agar surface.
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Using sterile tools, inoculate the agar plates with a uniform layer of the bacterial culture to ensure consistent results.
Step 4
Place a sterile paper disc soaked in Jelly Bush honey in the middle of an agar plate. Seal and label as Jelly.
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This step involves soaking a sterile paper disc in Jelly Bush honey and placing it in the center of one agar plate to assess its inhibitory effect on bacterial growth.
Step 5
Incubate the plates at 25°C for 48 hours.
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Incubate all plates at a consistent temperature of 25°C for 48 hours, allowing sufficient time for bacterial growth.
Step 6
Remove from incubator but do not open any of the plates after incubation.
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Carefully remove the plates from the incubator to avoid contamination while ensuring they remain sealed until analysis.
Step 7
Observe the control plate to check for any contaminating bacteria.
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Check the control plate for any unwanted bacterial growth to confirm effective sterilization during the experiment.
Step 8
For each agar plate, measure the zone of inhibition from edge to edge. Repeat the experiment more times and calculate an average.
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Measure the diameter of the area around the paper disc where bacteria have not grown, known as the zone of inhibition, and calculate averages based on multiple trials to ensure accuracy.
