The diagram below is a map of a bacterial plasmid showing ORI, the origin of DNA replication, and selected restriction endonuclease sites - VCE - SSCE Biology - Question 39 - 2019 - Paper 1
Question 39
The diagram below is a map of a bacterial plasmid showing ORI, the origin of DNA replication, and selected restriction endonuclease sites.
One plasmid was mixed wit... show full transcript
Worked Solution & Example Answer:The diagram below is a map of a bacterial plasmid showing ORI, the origin of DNA replication, and selected restriction endonuclease sites - VCE - SSCE Biology - Question 39 - 2019 - Paper 1
Step 1
Identify the restriction sites
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Answer
From the diagram, the restriction sites present are for EcoRI, BamHI, HincII, and PstI. The specific cuts of these enzymes should be identified based on the diagram.
Step 2
Calculate the number of cuts
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Answer
For EcoRI, BamHI, and HincII, we will count the number of sites cut. EcoRI cuts once, BamHI cuts once, and HincII cuts once. This gives a total of 3 restriction sites cut.
Step 3
Determine the number of DNA fragments produced
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Answer
When one restriction site is cut, it increases the number of DNA fragments. For n cuts, the number of resulting fragments is given by the formula:
extNumberoffragments=n+1
For 3 cuts, this results in:
3+1=4
Thus, the number of DNA fragments produced is 4.
Step 4
Select the correct answer option
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Answer
Based on the analysis, the answer is C: 4 restriction sites cut and 4 DNA fragments produced.
