A soil bacterium (Agrobacterium tumefaciens) infects roses and fruit trees, stunting their growth - VCE - SSCE Biology - Question 25 - 2007 - Paper 1
Question 25
A soil bacterium (Agrobacterium tumefaciens) infects roses and fruit trees, stunting their growth. A similar bacterium (A. radiobacter) was genetically modified to i... show full transcript
Worked Solution & Example Answer:A soil bacterium (Agrobacterium tumefaciens) infects roses and fruit trees, stunting their growth - VCE - SSCE Biology - Question 25 - 2007 - Paper 1
Step 1
removal of all plasmids.
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Answer
This option is not necessary because some plasmids are needed for the successful incorporation of the modified gene.
Step 2
removal of the antibiotic gene.
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Answer
This is incorrect. The antibiotic gene is not needed for the modification.
Step 3
destruction of the antibiotic resistance gene.
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Answer
This option is not required since the antibiotic resistance gene helps A. radiobacter survive in the presence of antibiotics.
Step 4
removal of the plasmid transfer gene.
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Answer
This is the correct choice. To ensure successful integration of the desired gene, the plasmid transfer gene must be removed to prevent unwanted gene transfer.
