A restriction enzyme cutting site may be present or absent in a particular 200 kb region of human chromosome 1 - VCE - SSCE Biology - Question 18 - 2005 - Paper 1
Question 18
A restriction enzyme cutting site may be present or absent in a particular 200 kb region of human chromosome 1.
cutting site
chromosome ... show full transcript
Worked Solution & Example Answer:A restriction enzyme cutting site may be present or absent in a particular 200 kb region of human chromosome 1 - VCE - SSCE Biology - Question 18 - 2005 - Paper 1
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Answer
The correct gel pattern would show three distinct bands: one at 200 kb representing the uncut fragment from the chromosome without the cutting site, one at 125 kb, and one at 75 kb coming from the chromosome with the cutting site. This indicates that the DNA has been cleaved at different positions, resulting from the heterozygous nature of the individual's DNA.