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Now consider a different length of double-stranded DNA with the sequence 5′ C T T A G C T A A T A C G A 3′ 3′ G A A T C G A T T A G C T 5′ Which enzyme(s) will cut this piece of DNA? A - VCE - SSCE Biology - Question 30 - 2013 - Paper 1
Question 30
Now consider a different length of double-stranded DNA with the sequence 5′ C T T A G C T A A T A C G A 3′ 3′ G A A T C G A T T A G C T 5′ Which enzyme(s) will cut... show full transcript
Worked Solution & Example Answer:Now consider a different length of double-stranded DNA with the sequence 5′ C T T A G C T A A T A C G A 3′ 3′ G A A T C G A T T A G C T 5′ Which enzyme(s) will cut this piece of DNA? A - VCE - SSCE Biology - Question 30 - 2013 - Paper 1
Step 1
Identify the restriction enzymes that can cut the provided DNA sequence
Answer
To determine which enzymes can cut the provided DNA sequence, we first need to analyze the restriction sites for common enzymes such as EcoRI, HindIII, and AluI.
- EcoRI recognizes the sequence GAATTC but this sequence is not present in the given DNA.
- HindIII recognizes the sequence AAGCTT, and this sequence is found in the DNA provided.
- AluI recognizes the sequence AA^CGTT, and this sequence can also be found in the provided DNA sequence.
Since both HindIII and AluI are present, the answer to which enzymes will cut this piece of DNA is C.