The following figures show two different methods used to sequence DNA - VCE - SSCE Biology - Question 7 - 2011 - Paper 1
Question 7
The following figures show two different methods used to sequence DNA.
Method 1
result – different colours
migration of DNA fragments
detector
laser beam
buffer
s... show full transcript
Worked Solution & Example Answer:The following figures show two different methods used to sequence DNA - VCE - SSCE Biology - Question 7 - 2011 - Paper 1
Step 1
A. method 1 requires 4 slots for the loading of the DNA.
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Answer
This statement is incorrect because Method 1 does not specify a requirement for 4 slots.
Step 2
B. method 2 uses a laser beam to separate the individual nucleotides.
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Answer
This statement is incorrect as Method 2 focuses on a gel system and does not mention the use of a laser beam.
Step 3
C. method 1 uses fluorescent dyes to distinguish between the different nucleotides.
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Answer
This statement is correct since Method 1 employs fluorescent dyes, allowing for differentiation between nucleotides as they migrate.
Step 4
D. method 2 requires the DNA to be loaded at the positive end on the sequencing gel.
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Answer
This statement may be misleading, as it does not clarify the loading procedure in detail, hence it cannot be deemed correct without further information.
