An enzyme is used to manipulate DNA as shown in the diagram below - VCE - SSCE Biology - Question 6 - 2022 - Paper 1
Question 6
An enzyme is used to manipulate DNA as shown in the diagram below.
5' ──────────────────────── 3'
A T C G A C T G A A
3' ──────────────────... show full transcript
Worked Solution & Example Answer:An enzyme is used to manipulate DNA as shown in the diagram below - VCE - SSCE Biology - Question 6 - 2022 - Paper 1
Step 1
This enzyme is an endonuclease.
96%
114 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Endonucleases are enzymes that cut DNA at specific internal locations. This statement suggests that the enzyme functions to cleave DNA strands, which aligns with the illustrated manipulation in the question.
Step 2
This enzyme could be used to join two DNA fragments together.
99%
104 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
This statement is false. The given diagram shows cutting of DNA strands, not joining.
Step 3
The action of this enzyme produces blunt ends on the two new fragments.
96%
101 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
This statement is also false because the diagram suggests that the enzyme generates sticky ends, not blunt ends.
Step 4
This enzyme breaks only hydrogen bonds between complementary base pairs.
98%
120 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
This statement is misleading. The enzyme mentioned shows the cleavage of the DNA backbone, not just the breaking of hydrogen bonds.
