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Incubation of this length of DNA in a tube containing A - VCE - SSCE Biology - Question 11 - 2002 - Paper 1
Question 11
Incubation of this length of DNA in a tube containing A. Spe I would result in three pieces of DNA. B. Hin dIII would result in two pieces of DNA. C. Spe I and Ec... show full transcript
Worked Solution & Example Answer:Incubation of this length of DNA in a tube containing A - VCE - SSCE Biology - Question 11 - 2002 - Paper 1
Step 1
A. Spe I would result in three pieces of DNA.
Answer
Spe I is a restriction enzyme that typically cuts DNA at specific sequences. To determine the number of pieces, we must know how many cutting sites exist in the given DNA length. If one cut is made, it increases the number of pieces by one. Thus, if there are two cutting sites for Spe I, it would result in three pieces of DNA.
Step 2
B. Hin dIII would result in two pieces of DNA.
Answer
Hin dIII cuts DNA at a specific sequence as well. If the target DNA has one recognizable cutting site for Hin dIII, it would indeed separate the DNA into two pieces. Therefore, this statement could potentially be accurate based on the cutting sites available.
Step 3
C. Spe I and Eco RI would result in five pieces of DNA.
Answer
When using two different enzymes, their respective cutting sites must be considered. If Spe I has two sites and Eco RI also has two separate sites, then the total number of cuts would be four. The formula used here is: number of cuts + 1 = total pieces, leading us to five pieces of DNA.
Step 4
D. Bgl II and Hin dIII would result in four pieces of DNA.
Answer
Similar to earlier examples, if Bgl II and Hin dIII each have two cutting sites in the DNA strand, then the combined cuts would yield a total of four pieces, which makes the understanding of the cutting process clear. Therefore, this statement should also be evaluated based on the expected cuts.