Plasmids of bacteria are used to transfer selected genes from one species to another - VCE - SSCE Biology - Question 25 - 2014 - Paper 1
Question 25
Plasmids of bacteria are used to transfer selected genes from one species to another.
The process can be represented as follows.
bacterial plasmid cut → foreign gen... show full transcript
Worked Solution & Example Answer:Plasmids of bacteria are used to transfer selected genes from one species to another - VCE - SSCE Biology - Question 25 - 2014 - Paper 1
Step 1
Cuts plasmid
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Answer
To cut the plasmid DNA, a restriction enzyme is needed. This enzyme recognizes specific sequences in the DNA and cleaves it to allow for the insertion of the foreign gene.
Step 2
Inserts genes
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Answer
To insert the foreign gene into the plasmid, DNA ligase is required. This enzyme facilitates the joining of the foreign gene with the plasmid DNA by creating phosphodiester bonds between the DNA fragments.
