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The tool used for joining the artificial gene to plasmid DNA at step T is A - VCE - SSCE Biology - Question 35 - 2013 - Paper 1
Question 35
The tool used for joining the artificial gene to plasmid DNA at step T is A. a primer. B. DNA ligase. C. DNA polymerase. D. gel electrophoresis.
Worked Solution & Example Answer:The tool used for joining the artificial gene to plasmid DNA at step T is A - VCE - SSCE Biology - Question 35 - 2013 - Paper 1
Step 1
Identify the correct tool for joining the artificial gene to plasmid DNA
Answer
The correct tool for joining the artificial gene to plasmid DNA is DNA ligase. DNA ligase is an essential enzyme that facilitates the joining of DNA strands together by forming phosphodiester bonds. In the context of molecular cloning, DNA ligase is used to join the DNA fragment (artificial gene) to the plasmid, thereby creating a recombinant DNA molecule.