Target DNA is to be inserted into a plasmid - VCE - SSCE Biology - Question 32 - 2020 - Paper 1
Question 32
Target DNA is to be inserted into a plasmid.
for a recombinant plasmid to be produced.
A. the plasmid sections and the target DNA must have blunt ends.
B. the targe... show full transcript
Worked Solution & Example Answer:Target DNA is to be inserted into a plasmid - VCE - SSCE Biology - Question 32 - 2020 - Paper 1
Step 1
A. the plasmid sections and the target DNA must have blunt ends.
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Answer
This statement is incorrect, as plasmid and target DNA can also have complementary sticky ends for effective ligation.
Step 2
B. the target DNA must come from the same species as the bacteria.
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Answer
This is not a requirement. Target DNA can originate from different species as long as the appropriate mechanisms for integration are applied.
Step 3
C. the plasmid and the target DNA must be cut by a polymerase.
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Answer
This statement is incorrect, as it is restriction enzymes that are used to cut the plasmid and target DNA, not a polymerase.
Step 4
D. DNA ligase is used to rejoin the sugar-phosphate sections of the plasmid and the target DNA.
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Answer
This statement is correct. DNA ligase is essential for joining the sugar-phosphate backbone of DNA fragments that have been cut.
