Two-dimensional electrophoresis of proteins is one technique used to study the products of gene action within a cell - VCE - SSCE Biology - Question 13 - 2011 - Paper 1
Question 13
Two-dimensional electrophoresis of proteins is one technique used to study the products of gene action within a cell. The first separation of proteins is based on pH... show full transcript
Worked Solution & Example Answer:Two-dimensional electrophoresis of proteins is one technique used to study the products of gene action within a cell - VCE - SSCE Biology - Question 13 - 2011 - Paper 1
Step 1
A. protein N has the same pH as protein P.
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Answer
This statement is supported by the electrophoresis results, as we can see that proteins with the same pH would remain at the same level in the tube of gel.
Step 2
B. protein T has a lower pH than protein M.
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Answer
According to the results, protein T is located lower on the pH separation compared to protein M, indicating that protein T indeed has a lower pH.
Step 3
C. protein Q has the same molecular size as protein R.
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The gel square separation shows that proteins Q and R are aligned at the same level, suggesting they have the same molecular size.
Step 4
D. protein M has a greater molecular size than protein S.
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The position of protein M in the gel indicates it is larger than protein S, confirming that protein M has a greater molecular size.
