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Use the following information to answer Questions 25–27 - VCE - SSCE Biology - Question 25 - 2015 - Paper 1
Question 25
Use the following information to answer Questions 25–27. To clone a gene of interest, the following four steps are performed: A plasmid is cut with a specific restri... show full transcript
Worked Solution & Example Answer:Use the following information to answer Questions 25–27 - VCE - SSCE Biology - Question 25 - 2015 - Paper 1
Step 1
What is the purpose of cutting the plasmid with a specific restriction enzyme?
Answer
Cutting the plasmid with a specific restriction enzyme opens it up for the insertion of the gene of interest. This step is crucial as it ensures that the ends of the plasmid are compatible with the ends of the gene being cloned, promoting successful ligation.
Step 2
What role does ligation play in the cloning process?
Answer
Ligation is the process where the gene of interest is covalently bonded to the plasmid. This step is vital for ensuring that the gene is effectively inserted into the plasmid, allowing for its replication within the bacterial host.
Step 3
Why are bacteria transferred to agar plates coated with ampicillin and arabinose?
Answer
Transferring bacteria to agar plates coated with ampicillin serves to select for those bacteria that have successfully taken up the plasmid, as only those expressing the antibiotic resistance gene can grow in the presence of the antibiotic. The inclusion of arabinose in some plates can induce expression of genes controlled by a promoter in the plasmid, aiding in the study of gene function.