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The optics of a confocal light microscope are constructed so that, when a specimen is viewed, only a single layer is observed and this is completely in focus - VCE - SSCE Biology - Question 4 - 2003 - Paper 1
Question 4
The optics of a confocal light microscope are constructed so that, when a specimen is viewed, only a single layer is observed and this is completely in focus. All ou... show full transcript
Worked Solution & Example Answer:The optics of a confocal light microscope are constructed so that, when a specimen is viewed, only a single layer is observed and this is completely in focus - VCE - SSCE Biology - Question 4 - 2003 - Paper 1
Step 1
A. Identify the Diagram Option
Answer
In a confocal light microscope, the design is intended to isolate a specific plane or layer of the specimen while eliminating out-of-focus light. Among the options provided in the diagram (A, B, C, D), we need to determine which configuration best reflects the principle of focusing on a single layer. The correct representation emphasizes a clear delineation of a layer, which aligns with the method of how confocal microscopy functions.
Step 2
B. Select the Correct Option
Answer
After analyzing the diagram options, option D clearly illustrates a single layer being highlighted, while options A, B, and C do not represent the principle accurately. Therefore, option D is the best representation of the single layer viewed in a confocal microscope.