Incubation of this length of DNA in a tube containing
A - VCE - SSCE Biology - Question 11 - 2002 - Paper 1
Question 11
Incubation of this length of DNA in a tube containing
A. SpeI would result in three pieces of DNA.
B. Hin dⅢ would result in two pieces of DNA.
C. SpeI and EcoRI ... show full transcript
Worked Solution & Example Answer:Incubation of this length of DNA in a tube containing
A - VCE - SSCE Biology - Question 11 - 2002 - Paper 1
Step 1
A. SpeI would result in three pieces of DNA.
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Answer
To determine the number of fragments produced by SpeI, we need to know its recognition site and cutting pattern on the provided DNA. SpeI generally produces three fragments when it recognizes its specific sites on the given DNA.
Step 2
B. Hin dⅢ would result in two pieces of DNA.
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Answer
Hin dⅢ typically recognizes a specific sequence and cuts the DNA at that location, leading to two fragments. Therefore, if the DNA has one Hin dⅢ recognition site, it would result in two pieces of DNA.
Step 3
C. SpeI and EcoRI would result in five pieces of DNA.
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Answer
The combination of SpeI and EcoRI would cut the DNA at their respective recognition sites. If both enzymes recognize unique sites and their cuts lead to a total of five fragments when combined, this option is viable.
Step 4
D. Bgl II and Hin dⅢ would result in four pieces of DNA.
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Answer
Using Bgl II alongside Hin dⅢ can result in four pieces of DNA, assuming that Bgl II cuts the DNA at additional recognition sites without overlapping with Hin dⅢ's cuts.
