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An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 3 - 2004 - Paper 1
Question 3
An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC). In both forms... show full transcript
Worked Solution & Example Answer:An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 3 - 2004 - Paper 1
Step 1
In terms of Rf and Ry, where Rf is the retention time in HPLC, component Z has the
Answer
Given that component Z bonds more strongly to the stationary phase than component Y, it will have a higher retention time in HPLC. This is because substances that interact more with the stationary phase have lower mobility and thus take longer to travel through the system. Therefore, for component Z:
- Rf would be lower than Ry, reflecting the stronger bonding to the stationary phase.
Hence, the correct choice is:
- C. lower Rf, higher Ry.