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Question 12
The identity of the fatty acids can be determined by measuring A. their retention times. B. the temperature of the column. C. the flow rate of the carrier gas.
Step 1
Answer
Fatty acids can be identified through their unique retention times in chromatography. Each type of fatty acid interacts differently with the stationary phase of the column, leading to distinct times for them to elute. This property can be used to differentiate between various fatty acids and establish their identity based on previously calibrated standards.
Step 2
Answer
The temperature of the column plays a significant role in the separation of fatty acids. By adjusting the temperature, one can influence the volatility of the compounds, hence optimizing their separation and potentially aiding in identification. However, it is the retention time that primarily identifies the fatty acids.
Step 3
Answer
The flow rate of the carrier gas affects the speed at which the fatty acids move through the column but does not inherently identify them. While it can influence retention times slightly, it is not a direct measure of the identity of the fatty acids. Thus, although it is a factor affecting the analysis, it does not primarily serve as a means of identification.
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