The separation and identification of proteins that can be used as disease markers is an exciting area of research - VCE - SSCE Chemistry - Question 20 - 2009 - Paper 1
Question 20
The separation and identification of proteins that can be used as disease markers is an exciting area of research. Researchers must separate and identify proteins th... show full transcript
Worked Solution & Example Answer:The separation and identification of proteins that can be used as disease markers is an exciting area of research - VCE - SSCE Chemistry - Question 20 - 2009 - Paper 1
Step 1
i. separate these molecules, then
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Answer
For the separation of proteins, high-performance liquid chromatography (HPLC) is an effective technique. It allows the separation of proteins based on their size, charge, or binding affinity.
Step 2
ii. accurately determine their molecular mass, and then
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Answer
Following the separation, mass spectrometry is a highly sensitive method for determining the molecular mass of proteins. It allows for precise measurement of the mass-to-charge ratio of charged particles.
Step 3
iii. determine their molecular structure.
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Answer
To determine the molecular structure of the proteins accurately, techniques such as Nuclear Magnetic Resonance (NMR) spectroscopy can be employed. NMR spectroscopy provides detailed information about the structure, dynamics, and interactions of proteins at the atomic level.
