The plate was dried, rotated through 90° in an anticlockwise direction and then placed in solvent F - VCE - SSCE Chemistry - Question 2 - 2012 - Paper 1
Question 2
The plate was dried, rotated through 90° in an anticlockwise direction and then placed in solvent F. The Rf values for each of the amino acids in solvent F are provi... show full transcript
Worked Solution & Example Answer:The plate was dried, rotated through 90° in an anticlockwise direction and then placed in solvent F - VCE - SSCE Chemistry - Question 2 - 2012 - Paper 1
Step 1
Name the amino acid that corresponds to spot 1.
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Answer
The Rf value of 0.51 in solvent G corresponds to both alanine and threonine. Therefore, the amino acid that corresponds to spot 1 is either alanine or threonine.
Step 2
Circle the spot on chromatogram II that represents alanine.
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Answer
In chromatogram II, you should circle the spot located on the left side marked with an Rf value of 0.21, which represents alanine.
Step 3
Explain, in terms of the data provided, why only three spots are present in chromatogram I while four spots are present in chromatogram II.
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Answer
In chromatogram I, which used solvent G, alanine and threonine appeared as the same spot since both had the same Rf value (0.51). In chromatogram II, which used solvent F, alanine and threonine had different Rf values and were separate, resulting in four distinct spots.
