Describe how the scientists could use aseptic techniques to transfer 0.3 cm³ of C - AQA - A-Level Biology - Question 8 - 2022 - Paper 1
Question 8
Describe how the scientists could use aseptic techniques to transfer 0.3 cm³ of C. difficile in liquid culture from a bottle onto an agar plate.
Worked Solution & Example Answer:Describe how the scientists could use aseptic techniques to transfer 0.3 cm³ of C - AQA - A-Level Biology - Question 8 - 2022 - Paper 1
Step 1
Use of Sterile Equipment
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Answer
To ensure that no contaminants are introduced, the scientists should use sterile equipment, such as a sterile pipette or inoculating loop, to transfer the liquid culture.
Step 2
Flame Sterilization
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Before transferring the culture, the scientists should flame the inoculating loop or the pipette tip to eliminate any potential microorganisms on the surface.
Step 3
Avoiding Contamination
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While transferring the 0.3 cm³ of C. difficile, the scientists should avoid touching any surfaces that could contaminate the culture, such as the sides of the agar plate.
