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Scientists investigated the effect of regular exercise on skeletal muscle fibres in mice - AQA - A-Level Biology - Question 3 - 2017 - Paper 1

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Scientists investigated the effect of regular exercise on skeletal muscle fibres in mice. The scientists compared the muscle fibres of mice after six weeks of regula... show full transcript

Worked Solution & Example Answer:Scientists investigated the effect of regular exercise on skeletal muscle fibres in mice - AQA - A-Level Biology - Question 3 - 2017 - Paper 1

Step 1

Succinic acid dehydrogenase is an enzyme used in the Krebs cycle. Suggest one reason for the difference in the staining between the muscle fibres of the control mice and the trained mice.

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Answer

The trained mice have a higher level of succinic acid dehydrogenase due to increased aerobic respiration compared to the control mice. This results in more intense staining in the muscle fibres of the trained mice.

Step 2

Explain why.

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Answer

The trained mice are able to sustain prolonged exercise due to enhanced muscle efficiency and increased oxidative capacity. Regular exercise improves the muscle's ability to utilize oxygen, which results in better aerobic energy production, allowing the trained mice to exercise for longer periods without fatigue.

Step 3

Using this information, calculate the mean diameter in µm (micrometres) of muscle fibres in this section of tissue.

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Answer

Given that the diameter of the field of view is equal to the diameter of 15 muscle fibres and the area of a field of view is 1.25 mm², we can relate the area to the diameter. The mean diameter can be calculated as:

d = rac{area}{n imes rac{ ext{π}}{4}}

where n is the number of muscle fibres:

Area = 1.25 ext{ mm}^2 = 1250 ext{ µm}^2

Thus, the mean diameter is:

rac{1250 ext{ µm}^2}{15} = 83.33 ext{ µm}

So, Mean Diameter = 83.33 µm.

Step 4

Describe two differences between these samples of muscle fibres.

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Answer

  1. Young mice have a larger range of muscle fibre diameters compared to adult mice, indicating greater variability in muscle fibre size during development.
  2. The mean diameter of muscle fibres in young mice is generally smaller than in adult mice, reflecting growth and maturation of the muscle tissue.

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