Describe how a sample of chloroplasts could be isolated from leaves - AQA - A-Level Biology - Question 5 - 2021 - Paper 1
Question 5
Describe how a sample of chloroplasts could be isolated from leaves.
1. Begin by breaking open the cells or tissue of the leaves. This can be accomplished by grindi... show full transcript
Worked Solution & Example Answer:Describe how a sample of chloroplasts could be isolated from leaves - AQA - A-Level Biology - Question 5 - 2021 - Paper 1
Step 1
1. Break open cells/tissue and filter
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Answer
Begin by breaking open the cells or tissue of the leaves. This can be accomplished by grinding or blending the leaves in a suitable solution.
Step 2
2. Use controlled solution
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Answer
Use a cold, isotonic buffer solution to prevent damage to the chloroplasts during the process. Ensure the solution has a controlled pH.
Step 3
3. Centrifuge to separate chloroplasts
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Next, centrifuge the mixture at a high speed to separate the chloroplasts, allowing them to settle at the bottom of the tube.
Step 4
4. Remove supernatant
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Finally, remove the supernatant containing cell debris and other components, leaving behind a purified sample of chloroplasts.
