A-Level AQA Biology Cell Recognition & the Immune System: Describe how a sample of chlorop

Question

Describe how a sample of chloroplasts could be isolated from leaves.

1. The first step is to break open the leaf cells. This can be achieved by grinding or blending the leaves in a cold, isotonic solution to maintain cell integrity.
2. Next, the mixture needs to be filtered to remove larger debris and other plant materials, allowing the liquid containing the chloroplasts to pass through.
3. After filtration, the solution should be centrifuged at a high speed to separate the chloroplasts from the supernatant. The chloroplasts will settle at the bottom of the centrifuge tube due to their size and density.
4. Finally, the liquid can be removed, leaving behind a pellet of chloroplasts that can be resuspended in a suitable buffer for further study.

Describe how a sample of chloroplasts could be isolated from leaves - AQA - A-Level Biology - Question 5 - 2021 - Paper 1

Worked Solution & Example Answer:Describe how a sample of chloroplasts could be isolated from leaves - AQA - A-Level Biology - Question 5 - 2021 - Paper 1

Describe how a sample of chloroplasts could be isolated from leaves.

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