Catalase is an enzyme - AQA - GCSE Biology - Question 2 - 2018 - Paper 1
Question 2
Catalase is an enzyme.
Catalase controls the following reaction:
hydrogen peroxide → water + oxygen
A student did an investigation on catalase activity.
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Worked Solution & Example Answer:Catalase is an enzyme - AQA - GCSE Biology - Question 2 - 2018 - Paper 1
Step 1
2.2 Circle the anomaly in Table 1.
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Answer
The anomaly in Table 1 is at 20 °C, where the maximum height of foam is recorded as 0.0 cm.
Step 2
2.4 What conclusion can be made as the temperature increases?
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Answer
As the temperature increases, the rate of reaction increases up to 30 °C. This can be determined by observing the trend in maximum height of foam with temperature.
Step 3
2.5 At which temperature was catalase denatured?
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Catalase was denatured at 60 °C, as indicated by the foam height returning to 0.0 cm.
Step 4
2.6 How could the investigation be improved to find a more precise value for the optimum temperature?
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The investigation could be improved by conducting the experiment at 30 °C, 35 °C, and 40 °C to gather more precise data around the optimum temperature.
Step 5
2.7 Describe how the student could investigate the effect of pH on the breakdown of starch by amylase.
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The student could investigate the effect of pH on the breakdown of starch by preparing a range of buffer solutions with different pH values. The method would involve measuring the breakdown of starch over time using iodine solution to detect the presence of starch. By keeping other variables constant, such as temperature and enzyme concentration, and measuring how the enzyme activity changes at each pH level, the effect of pH can be analyzed.