Photo AI
A student investigated the effect of enzyme concentration on the mass of product formed in one hour - Edexcel - GCSE Biology Combined Science - Question 3 - 2018 - Paper 1
Question 3
A student investigated the effect of enzyme concentration on the mass of product formed in one hour. Figure 3 shows the results of this investigation. (a) Complete ... show full transcript
Worked Solution & Example Answer:A student investigated the effect of enzyme concentration on the mass of product formed in one hour - Edexcel - GCSE Biology Combined Science - Question 3 - 2018 - Paper 1
Step 1
Complete the graph by plotting the points and drawing a line to show the trend in the data.
Answer
The graph should display all points plotted correctly within +/- 0.5 small square. A line indicating a steady increase, leveling off at a concentration of 30 arbitrary units and 40 grams of product formed, should connect the plotted points.
Step 2
Describe the effect that enzyme concentration has on the mass of product formed.
Answer
The mass of product formed increases as enzyme concentration increases. This trend continues until it levels off, where the mass of product formed remains constant, specifically at 30 arbitrary units of enzyme concentration and 40 grams of product.
Step 3
Calculate the ratio of enzyme concentration to product formed when the enzyme concentration is 5 arbitrary units.
Answer
Given the ratio of enzyme concentration to mass of product formed at 40 arbitrary units is 1:1, we can determine that at 5 arbitrary units, the ratio is:
Thus, the answer is 1:3.
Step 4
Explain why a temperature of 80°C was not used in this investigation.
Answer
A temperature of 80°C was not used because it would denature the enzyme pepsin, altering its structure and preventing it from catalyzing the reaction effectively. Higher temperatures can also change the shape of the enzyme, making it less effective or entirely inactive.