A student investigated the antibiotic properties of garlic - Edexcel - GCSE Biology - Question 5 - 2023 - Paper 1
Question 5
A student investigated the antibiotic properties of garlic.
Bacteria were spread on an agar plate.
A paper disc was soaked in garlic extract and placed on this agar ... show full transcript
Worked Solution & Example Answer:A student investigated the antibiotic properties of garlic - Edexcel - GCSE Biology - Question 5 - 2023 - Paper 1
Step 1
Suggest why there is an area with no bacterial growth.
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Answer
The area with no bacterial growth suggests that the garlic extract has antibiotic properties which effectively killed the bacteria in that region.
Step 2
Explain which agar plate, shown in Figure 10, has the paper disc containing the 100 mg/cm² garlic extract.
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Answer
Agar plate C is the one with the paper disc containing the 100 mg/cm² garlic extract, as it shows the largest clear zone with no microbial growth, indicating the highest antibacterial activity.
Step 3
Give one way the student could improve their results.
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One way the student could improve their results is by ensuring that the agar plates are incubated at the same temperature during the experiment.
Step 4
Calculate the area with no bacterial growth for agar plate B.
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To find the area, we use the formula for the area of a circle:
ext{Area} = rac{22}{7} r^2
Substituting the radius (r = 5 mm):
ext{Area} = rac{22}{7} imes (5^2) = rac{22}{7} imes 25 = 78.5 ext{ mm}^2
Step 5
State the reason for heating the spreader in the flame of a Bunsen burner before use.
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The spreader is heated to sterilise it, which helps to eliminate any bacteria or microorganisms present on the spreader.
Step 6
Give two other ways of working safely with microorganisms.
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Disinfect the work surface before starting the experiment.
Use protective gloves or a face mask when handling microorganisms.