A scientist was planning to compare the effectiveness of the antibiotic myxopyronin on two different species of bacteria - Edexcel - GCSE Biology - Question 10 - 2018 - Paper 1

To determine the effectiveness of myxopyronin on the two species of bacteria, the scientist should follow these steps:

1. Prepare the Agar Plates: Spread each of the two different species of bacteria onto separate agar plates to ensure they are evenly distributed.

2. Apply Myxopyronin Filter Discs: Place filter discs soaked in myxopyronin on the surface of the agar plates where the bacteria have been spread.

3. Incubate the Plates: Incubate the agar plates under appropriate conditions to allow bacterial growth and the antibiotic action to occur.

4. Measure the Zones of Inhibition: After incubation, observe the agar plates for zones of inhibition around the filter discs. Measure the diameter of these zones to assess the effectiveness of myxopyronin for each bacterial species.