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A student investigated the antibiotic properties of garlic - Edexcel - GCSE Biology - Question 5 - 2023 - Paper 1

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A student investigated the antibiotic properties of garlic. Bacteria were spread on an agar plate. A paper disc was soaked in garlic extract and placed on this agar ... show full transcript

Worked Solution & Example Answer:A student investigated the antibiotic properties of garlic - Edexcel - GCSE Biology - Question 5 - 2023 - Paper 1

Step 1

Suggest why there is an area with no bacterial growth.

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Answer

The area with no bacterial growth is likely due to the presence of the garlic extract, which has antimicrobial properties that inhibit or kill the bacteria.

Step 2

Explain which agar plate, shown in Figure 10, has the paper disc containing the 100 mg/cm² garlic extract.

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Answer

Agar plate C has the paper disc containing the 100 mg/cm² garlic extract because it shows the largest clear zone with no bacterial growth, indicating a stronger antibacterial effect compared to the lower concentrations.

Step 3

Give one way the student could improve their results.

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Answer

The student could use a control by including an agar plate without any garlic extract to compare the antibacterial effect.

Step 4

Calculate the area with no bacterial growth for agar plate B.

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Answer

The area of a circle is calculated using the formula: A=extπr2A = ext{π} r^2 Substituting the values, we have: A=3.14imes(5extmm)2=3.14imes25=78.5extmm2.A = 3.14 imes (5 ext{ mm})^2 = 3.14 imes 25 = 78.5 ext{ mm}^2.

Step 5

State the reason for heating the spreader in the flame of a Bunsen burner before use.

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Answer

The spreader is heated to sterilize it and kill any bacteria or microorganisms that may be present on the surface.

Step 6

Give two other ways of working safely with microorganisms.

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Answer

  1. Disinfect the work surface or area to prevent contamination.
  2. Work close to a Bunsen burner flame to create an updraft that helps to minimize the risk of airborne contamination.

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