As apples ripen, enzymes convert starch into sugars - Edexcel - GCSE Biology - Question 2 - 2023 - Paper 1
Question 2
As apples ripen, enzymes convert starch into sugars.
Devise a method to find the optimum pH of an enzyme that breaks down starch.
You may use standard laboratory equ... show full transcript
Worked Solution & Example Answer:As apples ripen, enzymes convert starch into sugars - Edexcel - GCSE Biology - Question 2 - 2023 - Paper 1
Step 1
Step 1: Prepare the Solutions
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Answer
Gather the starch solution, enzyme solution, iodine solution, and a range of pH solutions. Prepare the pH solutions to cover a range from acidic to basic (for example, pH 4 to pH 10).
Step 2
Step 2: Set Up the Experiment
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Answer
In a test tube, add a fixed amount of starch solution along with a specific pH solution. Record the pH level being tested.
Step 3
Step 3: Add the Enzyme
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Introduce a measured amount of enzyme solution to the test tube and mix gently. Allow the reaction to proceed for a specific time period.
Step 4
Step 4: Test for Sugar Production
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After the incubation period, add a few drops of iodine solution to the test tube. If the solution turns blue-black, starch is still present; if it remains brown, starch has been broken down into sugars.
Step 5
Step 5: Record and Analyze Results
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Answer
Repeat the experiment for each pH level tested. Record the color change observed with iodine for each test. Analyze the results to determine the pH at which the most effective breakdown of starch occurs, indicated by the absence of blue-black coloration.
