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Plant tissue culture is a technique used to produce plants, which is carried out under sterile conditions to prevent growth of microorganisms - Scottish Highers Biology - Question 4
Question 4
Plant tissue culture is a technique used to produce plants, which is carried out under sterile conditions to prevent growth of microorganisms. In an investigation t... show full transcript
Worked Solution & Example Answer:Plant tissue culture is a technique used to produce plants, which is carried out under sterile conditions to prevent growth of microorganisms - Scottish Highers Biology - Question 4
Step 1
Describe how unspecialised cells in the meristem differentiate into specialised cells.
Answer
Unspecialised cells in the meristem differentiate into specialised cells through a process where certain genes are expressed or switched on. This leads to the development of characteristics specific to that cell type, which allows them to take on their respective functions. Various factors, including environmental signals and hormonal influences, play a crucial role in this differentiation process.
Step 3
Suggest why shoot growth would be reduced if the investigation was set up in non-sterile conditions.
Answer
In non-sterile conditions, micro-organisms, bacteria, or fungi would grow, competing for nutrients, resources, and raw materials necessary for the plant shoots. This competition would limit the availability of essential resources, thereby reducing overall shoot growth. Additionally, these organisms may cause diseases that further inhibit plant development.
Step 4
Calculate the average increase in root length per µM of auxin between 0 and 6 µM.
Answer
To calculate the average increase:
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Identify the root length at 0 µM auxin: 0.4 mm.
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Identify the root length at 6 µM auxin: 1.6 mm.
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Calculate the increase in root length: 1.6 mm - 0.4 mm = 1.2 mm.
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Divide by the change in auxin concentration:
= 1.2 mm / 6 µM = 0.2 mm/µM.
So, the average increase in root length per µM of auxin is 0.2 mm/µM.
Step 5