An experiment was carried out to investigate the effect of different respiratory substrates on the rate of respiration in yeast (Saccharomyces cerevisiae) - Scottish Highers Biology - Question 12 - 2022
Question 12
An experiment was carried out to investigate the effect of different respiratory substrates on the rate of respiration in yeast (Saccharomyces cerevisiae).
Resazuri... show full transcript
Worked Solution & Example Answer:An experiment was carried out to investigate the effect of different respiratory substrates on the rate of respiration in yeast (Saccharomyces cerevisiae) - Scottish Highers Biology - Question 12 - 2022
Step 1
The same species of yeast was used.
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Answer
Using the same species of yeast ensures that any changes in the rate of respiration are due to the different substrates and not variations in yeast type.
Step 2
Five different respiratory substrates were used.
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This allows comparison across different substrates to determine their relative effectiveness in promoting respiration.
Step 3
Respiration rate was measured using a colorimeter.
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The use of a colorimeter provides a quantitative measure of the rate of respiration based on the color change of the dye.
Step 4
Resazurin dye was used.
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Resazurin dye acts as an indicator for the reduction reactions that occur during respiration, enabling visualization of the process.
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