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DNA recombinant technology can involve the insertion of a desired gene into a plasmid - Scottish Highers Biology - Question 16 - 2023
Question 16
DNA recombinant technology can involve the insertion of a desired gene into a plasmid. The diagram shows restriction sites on a chromosome containing the desired gen... show full transcript
Worked Solution & Example Answer:DNA recombinant technology can involve the insertion of a desired gene into a plasmid - Scottish Highers Biology - Question 16 - 2023
Step 1
The desired gene should be removed with
Answer
To remove the desired gene from the chromosome, one must consider the restriction sites available. The restriction enzyme HindIII is indicated at position H in the diagram. This enzyme cuts DNA at specific sequences, allowing the desired gene to be excised efficiently.
Among the options provided:
- A. HindIII is the correct choice as it enables the removal of the desired gene without introducing additional cuts through other restriction enzymes.
- B. EcoR1 and C. BamH1 would not be suitable as they do not optimally flank the desired gene in this configuration.
- D. BamH1 and HindIII represents an unnecessary complexity as HindIII alone suffices to remove the gene effectively.