Test tubes each containing an enzyme solution with different concentrations of substrate were incubated - Scottish Highers Biology - Question 9 - 2022
Question 9
Test tubes each containing an enzyme solution with different concentrations of substrate were incubated. After 10 minutes enzyme activity was measured.
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Worked Solution & Example Answer:Test tubes each containing an enzyme solution with different concentrations of substrate were incubated - Scottish Highers Biology - Question 9 - 2022
Step 1
A Inhibitor Y changes the shape of the active site.
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Answer
This statement is not supported by the data, as it pertains to non-competitive inhibition. Inhibitor Y's effects at different substrate concentrations do not indicate a change in the active site shape.
Step 2
B The optimum substrate concentration is 1.0 mol/l.
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Although enzyme activity is measured at 1.0 mol/l, the maximum observed activity of 0.5 units is also found at 0.8 mol/l. Thus, this statement may not be entirely accurate.
Step 3
C Inhibitor X binds to the active site.
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The data does not support this statement, as Inhibitor X shows a decrease in enzyme activity only at higher substrate concentrations, indicating possible allosteric inhibition or a different mechanism.
Step 4
D Inhibitor Y is a competitive inhibitor.
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This statement is supported by the data. Since Inhibitor Y's presence reduces enzyme activity but does not prevent it from reaching a similar maximum activity as at lower substrate levels, it behaves like a competitive inhibitor, competing with substrate for the active site.
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