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The table shows the effect of a competitive inhibitor on an enzyme's activity - Scottish Highers Human Biology - Question 7 - 2023
Question 7
The table shows the effect of a competitive inhibitor on an enzyme's activity. | Concentration of inhibitor (mM) | Enzyme activity (%) | |--------------------------... show full transcript
Worked Solution & Example Answer:The table shows the effect of a competitive inhibitor on an enzyme's activity - Scottish Highers Human Biology - Question 7 - 2023
Step 1
Predict the concentration of inhibitor when half of the active sites of the enzyme are occupied by inhibitor.
Answer
To find the concentration of inhibitor when half of the active sites are occupied, we need to identify the enzyme activity percentage that corresponds to this condition.
From the table, 100% activity is observed at 0.00 mM of inhibitor, and as the inhibitor concentration increases, the enzyme activity decreases.
If half of the active sites are occupied, we expect approximately 50% enzyme activity. Looking at the table:
- At 0.10 mM, the activity is 60%.
- At 0.20 mM, the activity is 40%.
Since 50% lies between these two values, we can use interpolation:
- The value we need is closer to 0.10 mM than 0.20 mM.
- Thus, we can estimate the concentration of inhibitor to be around 0.15 mM.
Therefore, the predicted concentration is 0.15 mM (Option A).