The diagram represents three steps during one cycle of the Polymerase Chain Reaction (PCR) - Scottish Highers Human Biology - Question 2 - 2022
Question 2
The diagram represents three steps during one cycle of the Polymerase Chain Reaction (PCR).
Step 1: single molecule of DNA
Step 2: separated DNA strands
Step 3: p... show full transcript
Worked Solution & Example Answer:The diagram represents three steps during one cycle of the Polymerase Chain Reaction (PCR) - Scottish Highers Human Biology - Question 2 - 2022
Step 1
Give values to describe the change in the temperature that occurs in step 2.
96%
114 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The temperature decreases to between 50°C and 65°C.
Step 2
Name the enzyme used in step 3.
99%
104 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The enzyme used in step 3 is DNA polymerase.
Step 3
Suggest an advantage of using a heat tolerant form of this enzyme during PCR.
96%
101 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
A heat tolerant enzyme will not denature during high temperatures, allowing multiple cycles of PCR without the enzyme needing to be replaced.
Step 4
Calculate the number of DNA molecules produced from a single molecule of DNA after 10 cycles of PCR.
98%
120 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
After 10 cycles of PCR, the number of DNA molecules produced is 210=1024.
Join the Scottish Highers students using SimpleStudy...
