The following steps occur during the Polymerase Chain Reaction (PCR) - Scottish Highers Human Biology - Question 3 - 2016
Question 3
The following steps occur during the Polymerase Chain Reaction (PCR).
1. Binding of primer
2. Replication of DNA
3. Heating of sample DNA
4. Separation of DNA stran... show full transcript
Worked Solution & Example Answer:The following steps occur during the Polymerase Chain Reaction (PCR) - Scottish Highers Human Biology - Question 3 - 2016
Step 1
1. Binding of primer
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Answer
This step occurs after the heating of the sample DNA, as the temperature is lowered to allow the primers to bind to the single-stranded DNA.
Step 2
2. Replication of DNA
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Following the binding of primers, the DNA polymerase synthesizes new strands of DNA during this step.
Step 3
3. Heating of sample DNA
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The process begins by heating the sample DNA to separate the strands, which is crucial for the next steps.
Step 4
4. Separation of DNA strands
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Answer
This is the initial step in PCR where the double-stranded DNA is denatured into two single strands.
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