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Investigating antibiotics & antiseptics Simplified Revision Notes

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Investigating antibiotics & antiseptics

Comparing the sizes of the inhibition zones compares the effectiveness of different antibiotics (or antiseptics or plant extracts)

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The effectiveness of the selected antibiotic or antiseptic can be quantified using the formula for the area of a circle, πr². Using the diagram below:

  1. For π, use the value 3.14. This value may vary slightly in specific questions.
  2. Measure the diameter of the zone of inhibition and divide it by 2 to obtain the radius (e.g., 10 ÷ 2 = 5 mm).
  3. Apply the equation: πr²
  • For example: 3.14 × 5² = 3.14 × 25 = 78.5 square millimeters.
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Calculate the sizes of the inhibition zones to compare results

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The larger the inhibition zone around the disc the more bacteria killed so the more effective the antibiotic is against the bacteria

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