It is important to use sterile apparatus when working with micro-organisms - Leaving Cert Biology - Question 7 - 2008
Question 7
It is important to use sterile apparatus when working with micro-organisms.
(a) (i) What is meant by sterile?
(ii) How may apparatus be sterilised?
(b) Answer t... show full transcript
Worked Solution & Example Answer:It is important to use sterile apparatus when working with micro-organisms - Leaving Cert Biology - Question 7 - 2008
Step 1
What is meant by sterile?
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Answer
Sterile means that the environment or material is free from micro-organisms, living things, or named organisms. This is essential in microbiology to prevent contamination.
Step 2
How may apparatus be sterilised?
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Answer
Apparatus can be sterilised through various methods, such as autoclaving, heating to high temperatures (e.g., over 100°C), boiling, streaming, or using UV light. It is important to remove any potential contaminants before use.
Step 3
Name the container in which you grew the leaf yeast.
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Answer
The leaf yeast was grown in a Petri dish.
Step 4
What was present in this container to provide food for the yeast?
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The container contained nutrient agar, which provides nutrients necessary for yeast growth.
Step 5
Describe how you put leaf yeast into the container.
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I carefully used sterile forceps to cut pieces of leaf and attached the upper surface of the leaf to the nutrient agar in the Petri dish. Adhering to safety precautions, I ensured that all surfaces were clean and free from contaminants.
Step 6
How long did it take for the leaf yeast to appear?
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The leaf yeast took more than 2 days, or approximately 24 hours, to appear.
Step 7
Describe the appearance of the leaf yeast in the container.
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The appearance of leaf yeast in the container was characterized by pink coloration or the development of dots, indicating the distribution of yeast colonies throughout the agar.
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