8 (a)
(i) Enzyme: Biological catalyst
(ii) Biomolecule enzymes made from: Protein
(b)
(i) Enzyme used: Named enzyme
(ii) Substrate used: Matching substrate
(iii) How temperature varied: Water bath(s) at different temperatures
(iv) How enzyme activity rate measured: Relevant change/per unit time
(v) How pH kept constant: Buffer (solution)
(vi) Result of investigation: e.g - Leaving Cert Biology - Question 8 - 2017
Question 8
8 (a)
(i) Enzyme: Biological catalyst
(ii) Biomolecule enzymes made from: Protein
(b)
(i) Enzyme used: Named enzyme
(ii) Substrate used: Matching substrate
(iii... show full transcript
Worked Solution & Example Answer:8 (a)
(i) Enzyme: Biological catalyst
(ii) Biomolecule enzymes made from: Protein
(b)
(i) Enzyme used: Named enzyme
(ii) Substrate used: Matching substrate
(iii) How temperature varied: Water bath(s) at different temperatures
(iv) How enzyme activity rate measured: Relevant change/per unit time
(v) How pH kept constant: Buffer (solution)
(vi) Result of investigation: e.g - Leaving Cert Biology - Question 8 - 2017
Step 1
Enzyme used: Named enzyme
96%
114 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The enzyme used in this investigation can be any specific enzyme relevant to the experiment being conducted, such as catalase, amylase, or lipase.
Step 2
Substrate used: Matching substrate
99%
104 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The substrate used should be one that specifically interacts with the chosen enzyme. For example, if catalase is used, the substrate would be hydrogen peroxide.
Step 3
How temperature varied: Water bath(s) at different temperatures
96%
101 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Temperature variation can be achieved using water baths set at different temperature intervals to measure the enzyme activity across a range of conditions.
Step 4
How enzyme activity rate measured: Relevant change/per unit time
98%
120 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The rate of enzyme activity can be measured by monitoring the rate of product formation or substrate consumption over a specified time period.
Step 5
How pH kept constant: Buffer (solution)
97%
117 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
A buffer solution is used to maintain a constant pH during the experiment, ensuring that fluctuations in pH do not affect the activity of the enzyme.
Step 6
Result of investigation: e.g. Increased activity as temperature increases or enzyme stops working after a certain temperature
97%
121 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The result of the investigation may indicate that enzyme activity increases with temperature until an optimal point is reached, after which the activity may decline due to denaturation.
Join the Leaving Cert students using SimpleStudy...
