7. (a) (i) What is an immobilised enzyme?
(ii) Give one advantage of using immobilised enzymes - Leaving Cert Biology - Question 7 - 2020
Question 7
7.
(a) (i) What is an immobilised enzyme?
(ii) Give one advantage of using immobilised enzymes.
(b) Answer the following questions in relation to an investigati... show full transcript
Worked Solution & Example Answer:7. (a) (i) What is an immobilised enzyme?
(ii) Give one advantage of using immobilised enzymes - Leaving Cert Biology - Question 7 - 2020
Step 1
What is an immobilised enzyme?
96%
114 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
An immobilised enzyme is an enzyme that is fixed, attached, or trapped in a specific location, typically on a solid support or within a gel. This allows the enzyme to remain in place during reactions.
Step 2
Give one advantage of using immobilised enzymes.
99%
104 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
One advantage of using immobilised enzymes is that they can be recovered and reused after the completion of the reaction, leading to cost savings and increased efficiency in industrial processes.
Step 3
Name the enzyme or cell that you used.
96%
101 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Yeast was used for the investigation.
Step 4
Describe how you immobilised that enzyme or cell.
98%
120 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
To immobilise the yeast, I used the following steps:
Prepare a sodium alginate solution.
Mix the yeast cells into the sodium alginate solution.
The mixture is then dropped into calcium chloride solution using a dropper or syringe, creating beads.
Allow the beads to set and leave them to rinse with water.
Step 5
Outline how you used the immobilised enzyme or cell to examine its application.
97%
117 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
The immobilised yeast cells were used in a fermentation process to convert a sugar substrate into alcohol. This involved mixing the beads with the sugar solution in a suitable vessel. The product formed was then measured to determine the efficiency of the reaction, along with monitoring control variables to ensure accurate results.
Join the Leaving Cert students using SimpleStudy...
