State a use of each of the following in the biology laboratory - Leaving Cert Biology - Question 7 - 2006
Question 7
State a use of each of the following in the biology laboratory.
(i) Biuret test (copper sulphate and sodium hydroxide solutions)..................................
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Worked Solution & Example Answer:State a use of each of the following in the biology laboratory - Leaving Cert Biology - Question 7 - 2006
Step 1
Use of Biuret test (copper sulphate and sodium hydroxide solutions)
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Answer
The Biuret test is used to detect the presence of proteins in a sample. The test works by reacting with the peptide bonds found in proteins, leading to a color change.
Step 2
Use of Benedict’s (or Fehling’s) test
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The Benedict’s test is used for the detection of reducing sugars in a solution, such as glucose or fructose. A color change in the reagent indicates the presence of these sugars.
Step 3
Investigation using IAA
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IAA (Indole-3-acetic acid) was used in an investigation to study its effect on plant growth.
The purpose was to determine how IAA influences the growth rate of plant cells in response to treatment.
Step 4
Investigation using Starch or skimmed milk agar plates
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Starch agar plates were used to investigate the activity of digestive enzymes.
The purpose was to observe the breakdown of starch and the formation of clear zones indicating enzyme activity.
Step 5
Investigation using Cold alcohol (ethanol)
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Cold alcohol was used for the isolation of DNA from biological samples.
The purpose was to effectively separate DNA from other cellular components.
Step 6
Investigation using Alkaline pyrogallol or anaerobic jar
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Alkaline pyrogallol was used to investigate conditions for germination in seeds.
The purpose was to remove oxygen from the environment to study the effects on germination, simulating anaerobic conditions.
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