In the course of your practical studies you prepared and examined with a microscope a transverse section (T.S.) of a dicotyledonous (dicot) plant stem - Leaving Cert Biology - Question 9 - 2019
Question 9
In the course of your practical studies you prepared and examined with a microscope a transverse section (T.S.) of a dicotyledonous (dicot) plant stem.
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Worked Solution & Example Answer:In the course of your practical studies you prepared and examined with a microscope a transverse section (T.S.) of a dicotyledonous (dicot) plant stem - Leaving Cert Biology - Question 9 - 2019
Step 1
What is meant by the term dicotyledonous?
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Answer
The term dicotyledonous refers to a group of flowering plants that have two seed leaves or cotyledons in their seeds. These plants typically exhibit a variety of characteristics such as a branching leaf venation and the presence of vascular bundles arranged in a ring.
Step 2
Name the plant you used.
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Answer
I used a buttercup (or rose or ivy) for my practical examination.
Step 3
Why is it important to use a very thin section when examining the tissues in the stem?
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A very thin section is crucial when examining tissues because it allows light to pass through effectively. This clarity enables detailed observation of cellular structures under the microscope, making it easier to identify different tissue types.
Step 4
Describe two steps you took to cut the stem safely.
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I ensured to use a microtome or a sharp scalpel to make a precise cut, keeping my fingers out of the cutting path.
I securely held the stem in a pith or firm support while cutting to prevent it from slipping.
Step 5
How did you transfer the section to the microscope slide?
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I used tweezers to carefully transfer the section to the microscope slide, ensuring not to touch the tissue directly.
Step 6
Name the part of the microscope you used to bring the section into a sharp focus.
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I used the fine focus knob to achieve a sharp focus on the specimen.
Step 7
Draw a labelled diagram of the section that you observed under the microscope.
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Answer
The diagram should include labels for various components such as the epidermis, vascular bundles, and other significant tissues observed in a ring form.
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