(a) (i) What is an enzyme?
A biological catalyst that speeds up a chemical reaction without being consumed in the process - Leaving Cert Biology - Question 8 - 2010
Question 8
(a) (i) What is an enzyme?
A biological catalyst that speeds up a chemical reaction without being consumed in the process.
(ii) Explain what is meant by the term ... show full transcript
Worked Solution & Example Answer:(a) (i) What is an enzyme?
A biological catalyst that speeds up a chemical reaction without being consumed in the process - Leaving Cert Biology - Question 8 - 2010
Step 1
What is an enzyme?
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Answer
An enzyme is a biological catalyst that accelerates chemical reactions in living organisms without being consumed during the reaction.
Step 2
Explain what is meant by the term pH.
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Answer
pH is a scale used to specify how acidic or basic a water-based solution is. It is measured on a range from 0 (very acidic) to 14 (very alkaline), with 7 being neutral. The pH value affects enzyme activity and overall biological processes.
Step 3
Name the enzyme you used in this investigation.
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The enzyme used in the investigation is Amylase.
Step 4
1. The substrate of this enzyme.
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Answer
The substrate of Amylase is Starch.
Step 5
2. The product of this enzyme.
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The product of Amylase is Maltose.
Step 6
Draw a labelled diagram of the apparatus you used in your investigation.
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A diagram should include a test tube containing starch solution, a way to measure pH (such as pH paper or a meter), and any additional equipment like pipettes or a water bath.
Step 7
How did you vary the pH?
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I varied the pH by adding different buffers, either by using acidic substances like hydrochloric acid or basic substances like sodium hydroxide.
Step 8
Name one factor you kept constant.
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One factor I kept constant was the temperature.
Step 9
How did you keep the named factor constant?
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The temperature was kept constant by using a water bath, ensuring that all reactions took place at the same temperature.
Step 10
Draw a graph, on the axes given below, to show the results of this investigation.
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The graph should display the rate of enzyme activity on the y-axis and pH on the x-axis, typically resulting in a bell-shaped curve indicating optimal enzyme activity at a specific pH level.
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