In one of your laboratory activities you isolated DNA from a plant tissue - Leaving Cert Biology - Question 7 - 2010
Question 7
In one of your laboratory activities you isolated DNA from a plant tissue.
(a) (i) Where in plant cells is DNA found?
(ii) What is meant by DNA profiling?
(b) (i)... show full transcript
Worked Solution & Example Answer:In one of your laboratory activities you isolated DNA from a plant tissue - Leaving Cert Biology - Question 7 - 2010
Step 1
Where in plant cells is DNA found?
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DNA is found in the nucleus, chloroplasts, or mitochondria of plant cells.
Step 2
What is meant by DNA profiling?
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DNA profiling refers to the analysis of an individual's DNA to compare patterns with others, often used in forensic science and paternity testing.
Step 3
Give one reason why you first chopped the plant material into very small pieces.
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Chopping the plant material into very small pieces increases the surface area, allowing for more efficient extraction of DNA.
Step 4
Explain why the detergent was used.
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Detergent was used to break down cell membranes, which helps to release the DNA into the solution.
Step 5
How was this mixture heated?
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The mixture was heated in a water bath to maintain a consistent temperature that aids in the breakdown of cellular components.
Step 6
Why was this mixture heated?
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Heating the mixture inactivates enzymes that could degrade the DNA, ensuring the integrity of the isolated DNA.
Step 7
What would happen to the DNA if the mixture was blended for longer than 3 seconds?
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Blending the mixture for longer than 3 seconds would likely shear the DNA, breaking it into smaller fragments and making it harder to isolate intact DNA.
Step 8
Why was protease added?
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Protease was added to digest proteins, particularly those that are bound to the DNA, allowing for a clearer extraction of the DNA.
Step 9
After filtration, where was the DNA of your plant tissue to be found?
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After filtration, the DNA would be found in the liquid or solution that passed through the filter.
Step 10
What should you do next to make the DNA visible?
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Next, add ice-cold ethanol or alcohol to the filtered solution, which will precipitate the DNA, making it visible.
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