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When isolating DNA from plant tissue: 1 - Leaving Cert Biology - Question iv - 2017
Question iv
When isolating DNA from plant tissue: 1. Give a reason for adding salt (NaCl) to the chopped plant tissue. 2. Why was the mixture containing the chopped plant tiss... show full transcript
Worked Solution & Example Answer:When isolating DNA from plant tissue: 1 - Leaving Cert Biology - Question iv - 2017
Step 1
Give a reason for adding salt (NaCl) to the chopped plant tissue.
Answer
Salt (NaCl) is added to the chopped plant tissue to help clump DNA together. It can also assist in separating proteins from DNA or even precipitating proteins and carbohydrates. This process is crucial as it aids in obtaining a clearer DNA sample for further analysis.
Step 2
Why was the mixture containing the chopped plant tissue, salt and washing-up liquid kept at 60 °C for a period of time?
Answer
The mixture was kept at 60 °C to denature DNases, which are enzymes that could otherwise destroy the DNA. By applying heat, these enzymes are rendered inactive, thus protecting the DNA during the extraction process.