In the course of your practical studies you prepared and examined with a microscope a transverse section (T.S.) of a dicotyledonous (dicot) plant stem - Leaving Cert Biology - Question 9 - 2019
Question 9
In the course of your practical studies you prepared and examined with a microscope a transverse section (T.S.) of a dicotyledonous (dicot) plant stem.
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Worked Solution & Example Answer:In the course of your practical studies you prepared and examined with a microscope a transverse section (T.S.) of a dicotyledonous (dicot) plant stem - Leaving Cert Biology - Question 9 - 2019
Step 1
What is meant by the term dicotyledonous?
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Answer
The term 'dicotyledonous' refers to a group of flowering plants (angiosperms) that have two seed leaves or cotyledons. These plants typically exhibit broad leaves, net-like veins, and flower parts in multiples of four or five. Examples include buttercup, sunflower, and roses.
Step 2
Name the plant you used.
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Answer
For the microscopic examination, I used the buttercup plant (Ranunculus spp.) as my specimen.
Step 3
Why is it important to use a very thin section when examining the tissues in the stem?
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Using a very thin section is crucial as it allows light to pass through effectively, enabling clear visibility of the individual cells and their structures. A thin section ensures that the tissue layers do not obscure one another, which is vital for accurate observation and analysis.
Step 4
Describe two steps you took to cut the stem safely.
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I used a microtome or a scalpel to ensure precision in cutting the stem, placing it on a stable surface to maintain control during the cut.
I cut the stem at an angle, away from my body, to minimize the risk of injury while ensuring that the section is even.
Step 5
How did you transfer the section to the microscope slide?
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I carefully used a paintbrush or forceps to gently lift the thin section and place it onto the microscope slide, avoiding any damage to the delicate tissues.
Step 6
Name the part of the microscope you used to bring the section into a sharp focus.
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I used the fine focus knob on the microscope to achieve a sharp and clear image of the section.
Step 7
Draw a labelled diagram of the section that you observed under the microscope.
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The diagram should illustrate a cross-section of the stem, showing the epidermis, vascular bundles, and pith. Clearly label the vascular bundles as xylem and phloem, along with other structures such as the cortex and epidermis.
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